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先玉335父本PH4CV专利文件（摘译）
农学人 译 2010-9-27
United States Patent 6,897,363
美国专利6,897,363
Inbred maize line PH4CV
玉米自交系PH4CV
Abstract
摘要
An inbred maize line, designated PH4CV, the plants and seeds of inbred
maize line PH4CV, methods for producing a maize plant, either inbred
or hybrid, produced by crossing the inbred maize line PH4CV with
another maize plant, and hybrid maize seeds and plants produced by
crossing the inbred line PH4CV with another maize line or plant and to
methods for producing a maize plant containing in its genetic material
one or more transgenes and to the transgenic maize plants produced by
that method. This invention also relates to inbred maize lines derived
from inbred maize line PH4CV, to methods for producing other inbred
maize lines derived from inbred maize line PH4CV and to the inbred
maize lines derived by the use of those methods.
一种名为PH4CV的玉米自交系、玉米自交系PH4CV的植株和种子、用玉米自交系
PH4CV与其它玉米植株杂交产生玉米植株（不管是自交系还是杂交种）的方法、
用自交系PH4CV与其它玉米品系或植株杂交所产生的杂交玉米种子和植株、产生
在其遗传物质中含有一个或多个转基因的玉米植株的方法、以及以这种方法产生
的转基因玉米植株。本发明还涉及由玉米自交系PH4CV衍生出来的玉米自交系、
产生由玉米自交系PH4CV衍生出其它玉米自交系的方法、以及利用这些方法衍生
出来的玉米自交系。
Inventors: Barker; Thomas Charles (York, NE)
发明人：Barker; Thomas Charles (York, NE)
Assignee: Pioneer Hi-Bred International, Inc. (Johnston, IA)
专利权人：Pioneer Hi-Bred International, Inc. (Johnston, IA)
Appl. No.: 10/271,942
申请号：10/271,942
Filed: October 15, 2002
受理日期：2002年10月15日
Current U.S. Class: 800/320.1; 435/412; 800/275; 800/278; 800/279;
800/281; 800/298; 800/300.1; 800/301; 800/302; 800/303
现有美国类别：800/320.1; 435/412; 800/275; 800/278; 800/279; 800/281;
800/298; 800/300.1; 800/301; 800/302; 800/303
Current International Class: A01H 5/10 (20060101); A01H 001/00; A01H
004/00; A01H 005/00; A01H 005/10; C12N 015/82
现有国际类别：A01H 5/10 (20060101); A01H 001/00; A01H 004/00; A01H
005/00; A01H 005/10; C12N 015/82
Field of Search: 800/320.1,266,275,278,279,284,298,300.1,301,302,303
435/412
检索领域：800/320.1,266,275,278,279,284,298,300.1,301,302,303 435/412
……
Claims
权利要求
What is claimed is:
权利要求是：
1. A seed of maize inbred line designated PH4CV, representative seed
of said line having been deposited under ATCC Accession No. PTA-4673.
名称为PH4CV的玉米自交系的种子，所指的自交系的标准种子已保存在美国生物
标本收藏中心（ATCC），登记号为No. PTA-4673。
2. A maize plant, or a part thereof, produced by growing the seed of
claim 1.
种植权利要求1的种子所产生的玉米植株或器官。
3. The maize plant of claim 2, wherein said plant has been detasseled.
已被去雄的权利要求2的玉米植株。
4. A tissue culture of regenerable cells produced from the plant of
claim 2.
由权利要求2的植株所产生的再生细胞的组织培养。
5. A protoplast produced from the tissue culture of claim 4.
由权利要求4的组织培养所产生的原生质体。
6. The tissue culture of claim 4, wherein cells of the tissue culture
are produced from a tissue selected from the group consisting of leaf,
pollen, embryo, root, root tip, anther, silk, flower, kernel, ear, cob,
husk and stalk.
权利要求4的组织培养，这里组织培养的细胞由以下组织产生：叶片、花粉、胚
胎、根系、根尖、花药、花丝、花、籽粒、穗、穗轴、包叶、茎秆。
7. A maize plant regenerated from the tissue culture of claim 4, said
plant having all the morphological and physiological characteristics
of inbred line PH4CV, representative seed of said inbred line having
been deposited under ATCC Accession No. PTA-4673.
权利要求4的组织培养再生的玉米植株，该植株具有自交系PH4CV的所有形态和生
理性状，所指的自交系的标准种子已保存在美国生物标本收藏中心（ATCC），登
记号为No. PTA-4673。
8. A method for producing an F1 hybrid maize seed, comprising crossing
the plant of claim 2 with a different maize plant and harvesting the
resultant F1 hybrid maze seed.
生产F1代杂交玉米种子的方法，即将权利要求2的植株与不同的玉米植株杂交，
收获所产生的F1代玉米杂交种子。
9. A method of producing a male sterile maize plant comprising
transforming the maize plant of claim 2 with a nucleic acid molecule
that confers male sterility.
产生雄性不育玉米植株的方法，即用可导致雄性不育的核酸分子转移权利要求2
的玉米植株。
10. A male sterile maize plant produced by the method of claim 9.
用权利要求9的方法产生的雄性不育玉米植株。
11. A method of producing an herbicide resistant maize plant
comprising transforming the maize plant of claim 2 with a transgene
that confers herbicide resistance.
产生抗除草剂玉米植株的方法，即用可导致除草剂抗性的转基因转移权利要求2
的玉米植株。
12. An herbicide resistant maize plant produced by the method of claim
11.
用权利要求11的方法产生的抗除草剂玉米植株。
13. The maize plant of claim 12, wherein the transgene confers
resistance to an herbicide selected from the group consisting of
imidazolinone, sulfonylurea, glyphosate, glufosinate,
L-phosphinothricin, triazine and benzonitrile.
权利要求12的玉米植株，其中被转移的基因对咪唑啉酮类、磺酰脲类、草甘膦、
草铵膦、L-草胺膦、三嗪和苯甲腈等除草剂具有抗性。
14. A method of producing an insect resistant maize plant comprising
transforming the maize plant of claim 2 with a transgene that confers
insect resistance.
产生抗虫玉米植株的方法，即用可导致抗虫性的转基因转移权利要求2的玉米植
株。
15. An insect resistant maize plant produced by the method of claim 14.
用权利要求14的方法产生的抗虫玉米植株。
16. The maize plant of claim 15, wherein the transgene encodes a
Bacillus thuringiensis endotoxin.
权利要求15的玉米植株，其中的转基因可以编码Bt内毒素。
17. A method of producing a disease resistant maize plant comprising
transforming the maize plant of claim 2 with a transgene that confers
disease resistance.
产生抗病玉米植株的方法，即用可导致抗病性的转基因转移权利要求2的玉米植
株。
18. A disease resistant maize plant produced by the method of claim 17.
用权利要求17的方法产生的抗病玉米植株。
19. A method of producing a maize plant with decreased phytate content
comprising transforming the maize plant of claim 2 with a transgene
encoding phytase.
产生低植酸酶含量玉米植株的方法，即用编码植酸酶的转基因转移权利要求2的
玉米植株。
20. A maize plant with decreased phytate content produced by the
method of claim 19.
用权利要求19的方法产生的低植酸酶含量的玉米植株。
21. A method of producing a maize plant with modified fatty acid
metabolism or modified carbohydrate metabolism comprising transforming
the maize plant of claim 2 with a transgene encoding a protein
selected from the group consisting of fructosyltransferase,
levansucrase, alpha-amylase, invertase and starch branching enzyme or
encoding an antisense of stearyl-ACP desaturase.
产生修饰的脂肪酸代谢或碳水化合物代谢玉米植株的方法，即用编码果糖转移酶、
果聚糖蔗糖酶、α-淀粉酶、转化酶和淀粉分支酶之一，或编码反义stearyl-ACP
desaturase的蛋白质的转基因转移权利要求2的玉米植株。
22. A maize plant, with modified fatty acid metabolism or modified
carbohydrate metabolism, produced by the method of claim 21.
用权利要求22的方法产生的具有修饰的脂肪酸代谢或修饰的碳水化合物代谢过程
的玉米植株。
23. The maize plant of 22 wherein the transgene confers a trait
selected from the group consisting of waxy starch and increased
amylose starch.
权利要求22的玉米植株，其转基因导致蜡质淀粉或高直链淀粉。
24. A maize plant, or part thereof, having all the physiological and
morphological characteristics of the inbred line PH4CV, representative
seed of said inbred line having been deposited under ATCC Accession No.
PTA-4673.
具有自交系PH4CV的所有生理和形态性状的玉米植株或器官，所指的自交系的标
准种子已保存在美国生物标本收藏中心（ATCC），登记号为No. PTA-4673。
25. A method of introducing a desired trait into maize inbred line
PH4CV comprising: (a) crossing PH4CV plants grown from PH4CV seed,
representative seed of which has been deposited under ATCC Accession No.
PTA-4673, with plants of another maize line that comprise a desired
trait to produce F1 progeny plants, wherein the desired trait is
selected from the group consisting of male sterility, herbicide
resistance, insect resistance, disease resistance and waxy starch; (b)
selecting F1 progeny plants that have the desired trait to produce
selected F1 progeny plants; (c) crossing the selected progeny plants
with the PH4CV plants to produce backcross progeny plants; (d)
selecting for backcross progeny plants that have the desired trait and
physiological and morphological characteristics of maize inbred line
PH4CV listed in Table 1 to produce selected backcross progeny plants;
and (e) repeating steps (c) and (d) three or more times in succession
to produce selected fourth or higher backcross progeny plants that
comprise the desired trait and all of the physiological and
morphological characteristics of maize inbred line PH4CV listed in
Table 1 as determined at the 5% significance level when grown in the
same environmental conditions.
将理想性状导入玉米自交系PH4CV的方法，包括：（1）用由PH4CV种子（标准种
子已保存在美国生物标本收藏中心（ATCC），登记号为No. PTA-4673）长成的
PH4CV植株，与其他具有某个理想性状（如雄性不育、抗除草剂、抗虫、抗病和
蜡质淀粉）的玉米品系的植株杂交，以产生F1代植株；（2）选择具有理想性状
的F1代植株以得到当选F1代植株；（3）用当选F1代植株与PH4CV植株回交以得到
回交后代植株；（4）选择具有理想性状和表1所列的玉米自交系PH4CV的生理和
形态性状的回交后代，以得到当选回交后代植株；（5）重复（3）和（4）连续3
次或以上，以得到当选第4代或更高世代的具有理想性状和表1所列的玉米自交系
PH4CV的全部生理和形态性状（以种植在相同环境条件下5%的显著水平决定）的
回交后代植株。
26. A plant produced by the method of claim 25, wherein the plant has
the desired trait and all of the physiological and morphological
characteristics of maize inbred line PH4CV listed in Table 1 as
determined at the 5% significance level when grown in the same
environmental conditions.
用权利要求25的方法产生的植株，该植株具有理想性状和表1所列的玉米自交系
PH4CV的全部生理和形态性状（以种植在相同环境条件下5%的显著水平决定）。
27. The plant of claim 26 wherein the desired trait is herbicide
resistance and the resistance is conferred to an herbicide selected
from the group consisting of imidazolinone, sulfonylurea, glyphosate,
glufosinate, L-phosphinothricin, triazine and benzonitrile.
权利要求26的植株，其理想性状是除草剂抗性，所抗的除草剂是咪唑啉酮类、磺
酰脲类、草甘膦、草铵膦、L-草胺膦、三嗪和苯甲腈之一。
28. The plant of claim 26 wherein the desired trait is insect
resistance and the insect resistance is conferred by a transgene
encoding a Bacillus thuringiensis endotoxin.
权利要求26的植株，其理想性状是抗虫性，这抗虫性是指由编码Bt内毒素的转基
因产生的抗性。
29. The plant of claim 26 wherein the desired trait is male sterility
and the trait is conferred by a cytoplasmic nucleic acid molecule that
confers male sterility.
权利要求26的植株，其理想性状是雄性不育性，该性状是由可导致雄性不育性的
细胞质核酸分子所产生。
30. A method of modifying fatty acid metabolism, phytic acid
metabolism or carbohydrate in maize inbred line PH4CV comprising: (a)
crossing PH4CV plants grown from PH4CV seed, representative seed of
which has been deposited under ATCC Accession No. PTA-4673, with
plants of another maize line that comprise a nucleic acid molecule
encoding an enzyme selected from the group consisting of phytase,
fructosyltransferase, levansucrase, alpha-amylase, invertase and
starch branching enzyme or encoding an antisense of stearyl-ACP
desaturase; (b) selecting F1 progeny plants that have said nucleic
acid molecule to produce selected F1 progeny plants; (c) crossing the
selected progeny plants with the PH4CV plants to produce backcross
progeny plants; (d) selecting for backcross progeny plants that have
said nucleic acid molecule and physiological and morphological
characteristics of maize inbred line PH4CV listed in Table 1 to
produce selected backcross progeny plants; and (e) repeating steps (c)
and (d) three or more times in succession to produce selected fourth
or higher backcross progeny plants that comprise said nucleic acid
molecule and have all of the physiological and morphological
characteristics of maize inbred line PH4CV listed in Table 1 as
determined at the 5% significance level when grown in the same
environmental conditions.
修饰玉米自交系PH4CV的脂肪酸代谢、植酸代谢、或碳水化合物代谢的方法，包
括：（1）用由PH4CV种子（标准种子已保存在美国生物标本收藏中心（ATCC），
登记号为No. PTA-4673）长成的PH4CV植株，与其他具有编码下述酶（植酸酶、
果糖转移酶、果聚糖蔗糖酶、α-淀粉酶、转化酶和淀粉分支酶，或编码反义
stearyl-ACP desaturase）之一的核酸分子的其他玉米品系的植株杂交；（2）
选择具有理所述核酸分子的F1代植株以得到当选F1代植株；（3）用当选后代植
株与PH4CV植株回交以得到回交后代植株；（4）选择具有所述核酸分子和表1所
列的玉米自交系PH4CV的生理和形态性状的回交后代，以得到当选回交后代植株；
（5）重复（3）和（4）连续3次或以上，以得到当选第4代或更高世代的具有所
述核酸分子和表1所列的玉米自交系PH4CV的全部生理和形态性状（以种植在相同
环境条件下5%的显著水平决定）的回交后代植株。
31. A plant, with modified fatty acid metabolism, modified phytic acid
metabolism, or modified carbohydrate metabolism, produced by the
method of claim 30, wherein the plant comprises the nucleic acid
molecule and has all of the physiological and morphological
characteristics of maize inbred line PH4CV listed in Table 1 as
determined at the 5% significance level when grown in the same
environmental conditions.
用权利要求30的方法产生的、具有修饰的脂肪酸代谢、修饰的植酸代谢、或修饰
的碳水化合物代谢的植株，该植株具有核酸分子和表1所列的玉米自交系PH4CV的
全部生理和形态性状（以种植在相同环境条件下5%的显著水平决定）。
……
附注：学好英文和学好植物遗传育种学后再来讨论转基因！既不懂农学，英文又
半生不熟，怎么能读懂美国的专利文献？先玉335父本PH4CV的专利文献非常严谨，
只要真懂英文，哪怕不懂农学，也不会认为它是“属于转基因（或含有转基因成
分）玉米品种”，只是以专利的形式要求其他个人或组织不得以PH4CV为材料进
行转基因。（2010-9-29）
(XYS20101001)
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